An isotope dilution mass spectrometric measurement procedure has the potential of being a very good reference measurement procedure, but is not a “definitive” one
نویسندگان
چکیده
منابع مشابه
Measurement of urea in human serum by isotope dilution mass spectrometry: a reference procedure.
BACKGROUND A reference measurement procedure is needed to demonstrate the traceability of results of urea measurements in human serum. We developed a measurement procedure using the principle of isotope dilution gas chromatography/mass spectrometry. METHODS [(13)C,(15)N(2)]Urea as internal standard was added to a serum sample and equilibrated with endogenous nonlabeled urea. For the preparati...
متن کاملReference measurement procedure for total glycerides by isotope dilution GC-MS.
BACKGROUND The CDC's Lipid Standardization Program established the chromotropic acid (CA) reference measurement procedure (RMP) as the accuracy base for standardization and metrological traceability for triglyceride testing. The CA RMP has several disadvantages, including lack of ruggedness. It uses obsolete instrumentation and hazardous reagents. To overcome these problems the CDC developed an...
متن کاملP 84: Daily Nutrition has a Grand but Inapparent Effect on the Procedure of Inflammatory Dementia
Alzheimer is age-related, progressive brain degeneration disease. It destroys memory slowly. The number of people living with AD is increasing as fast as the count of aged population and extension of bad nutrition habits. The main cause of AD is not clearly known, but prior studies noted the importance of inflammation in Tau hyperphosphorylation and failure of microglia to clear AB peptide accu...
متن کاملStable-isotope dilution gas chromatography-mass spectrometric measurement of 3-hydroxyglutaric acid, glutaric acid and related
We developed a simple and sensitive stable-isotope dilution method for the quantification of 3-hydroxyglutaric acid (3HGA) and glutaric acid (GA) in body fluids. In our method, tertbutyldimethylsilyl derivatives of 3HGA and GA were measured with a conventional electron-impact ionization mode in gas chromatography-mass spectrometry. The control values for 3HGA in nmol/ml were 0.15 ± 0.08 (serum;...
متن کاملDevelopment of a candidate reference measurement procedure for the analysis of cortisol in human serum samples by isotope dilution-gas chromatography-mass spectrometry.
A candidate reference measurement procedure involving isotope dilution coupled with gas chromatography-mass spectrometry (GC-MS) has been developed and critically evaluated. An isotopically labeled internal standard, cortisol-d(2), was added to a serum sample. After equilibration, solid-phase extractions (SPE) for sample preparation and derivatization with heptafluorobutyric anhydride (HFBA) we...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Accreditation and Quality Assurance
سال: 2010
ISSN: 0949-1775,1432-0517
DOI: 10.1007/s00769-010-0657-x